Covers the basic techniques of plant and animal cell culture. Plant unit includes media preparation isolation of explants and establishment of callus from suspension cultures, growth factor bioassays, regeneration of whole plants from tissue and plant genetic engineering techniques. Mammalian cell unit includes media preparation, maintenance of cultured cells, transfection of cultured cells, cloning, monoclonal antibody production, and ELISA assays. Lab included. Prerequisite: 10-007-115 or consent of instructor.